کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2796024 1568747 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Immunization of mice with T cell-dependent antigens promotes IL-6 and TNF-α production in muscle cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی علوم غدد
پیش نمایش صفحه اول مقاله
Immunization of mice with T cell-dependent antigens promotes IL-6 and TNF-α production in muscle cells
چکیده انگلیسی

IL-6 and TNF-α are proinflammatory cytokines involved in various inflammatory or non-inflammatory disorders characterized by muscle wasting. While infiltrating leukocytes are known to be the major source of these cytokines, it is unclear whether muscle cells also contribute to local inflammation. In this study, we first showed that cultured muscle cells and naive mouse muscle tissue were capable of producing IL-6 and TNF-α. We demonstrated an increased expression of IL-6 and TNF-α on muscle sections of C57BL/6J mice immunized with acetylcholine receptor (AChR) in the complete Freund’s adjuvant (CFA) or with CFA only. In the presence of IL-6 or TNF-α, cultured AChR-expressing mouse (G-8) and human (TE671) skeletal muscle cells showed significantly decreased α-bungarotoxin-binding sites as measured by cellular ELISA. Moreover, IL-6- or TNF-α-treated muscle cells displayed a considerable increase in apoptotic cell ratios. Collectively, this study suggests a direct role for these two cytokines in muscle cell destruction and a possibility of muscle cell damage via an autocrine fashion.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cytokine - Volume 35, Issues 1–2, July 2006, Pages 100–106
نویسندگان
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