کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2800154 | 1568904 | 2014 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Do photoperiod and endocrine disruptor 4-tert-octylphenol effect on spermatozoa of bank vole (Clethrionomys glareolus)?
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
علوم غدد
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Do photoperiod and endocrine disruptor 4-tert-octylphenol effect on spermatozoa of bank vole (Clethrionomys glareolus)? Do photoperiod and endocrine disruptor 4-tert-octylphenol effect on spermatozoa of bank vole (Clethrionomys glareolus)?](/preview/png/2800154.png)
چکیده انگلیسی
Photoperiod is an environmental signal that controls physiology and behavior of all organisms. Bank voles, which are seasonal breeders, are stimulated to reproduce by the long photoperiod associated with spring and summer. To date, physiology of bank vole spermatozoa has not been explored, although they constitute an interesting model for examining the relationship between photoperiod and xenoestrogen on spermatozoa function. In an attempt to evaluate the acute effect of 4-tert-octylphenol (OP) an in vitro system was used. Spermatozoa isolated from the cauda epididymidies of long-day (LD; 18Â h light: 6Â h darkness) and short-day (SD; 6Â h light: 18Â h darkness) bank voles were treated with two OP concentrations (10â4 M and 10â8Â M, respectively). OP-treated spermatozoa were used for the examination of motility parameters (computer-assisted semen analyzer CEROS), acrosome integrity (Commassie blue staining), cAMP production (immunoenzymatic assay) and cell viability (flow-cytometry analysis). The study revealed the photoperiod-dependent effect of short OP-treatment on motility parameters of vole spermatozoa. In LD spermatozoa, an increase of velocities: (curvilinear velocity [VCL], average path velocity [VAP] straight line velocity [VSL]) and head activity (amplitude of the lateral head displacement, [ALH]) was found. Interestingly, in SD spermatozoa opposite effect on VCL, VAP, VSL and ALH was observed, however only after treatment with 10â4Â M OP. The dose-dependent influence of OP upon acrosome integrity, as well as cAMP levels, in relation to the reproductive status of voles was observed. Moreover, OP exposure affected spermatozoa morphology rather than spermatozoa viability.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: General and Comparative Endocrinology - Volume 201, 15 May 2014, Pages 21-29
Journal: General and Comparative Endocrinology - Volume 201, 15 May 2014, Pages 21-29
نویسندگان
Malgorzata Kotula-Balak, Pawel Grzmil, Katarzyna Chojnacka, Katarzyna Andryka, Barbara Bilinska,