Teleost maturation-inducing hormone, 17,20β-dihydroxypregn-4-en-3-one, peaks after spawning in Tinca tinca

During an eight month study of the reproductive cycle in two age groups, and in both sexes, of tench (Tinca tinca L.), it was found that plasma concentrations of the presumptive ‘maturation inducing hormone (MIH)’ 17,20β-dihydroxypregn-4-en-3-one (17,20β-P) did not reach a peak during the spawning season, but as much as two months after spawning had ceased. The cessation of the spawning season was confirmed by histological examination of the gonads and by measurement of 11-ketotestosterone and 17β-estradiol in the plasma of males and females, respectively. Measurements were also made of the ‘alternative MIH’ 17,20β,21-trihydroxypregn-4-en-3-one in the older fish. However, this steroid did not show the same pattern as 17,20β-P. An assessment was made of the prevalence of primary spermatocytes in the testes of post-spawned fish – to test an alternative hypothesis that 17,20β-P might be involved in the stimulation of meiosis. However, there was no evidence for any increase in testis differentiation post-spawning. In fact the testes became increasingly undifferentiated as the autumn progressed. The role, if any, of this ‘unseasonal’ peak of 17,20β-P production remains to be determined.

► Peak production of 17,20β-P is not associated with spawning time in T. tinca.
► 17,20β,21-P did not show the same pattern as 17,20β-P in adult T. tinca.
► 17,20β-P peak is not associated with the initiation of gonadal meiosis in T. tinca.
► The dogma of elevated 17,20β-P levels with maturation-spermiation is challenged.