Postprandial effects on appetite-related neuropeptide expression in the brain of Atlantic salmon, Salmo salar

Following feeding of a single meal to Atlantic salmon, the temporal changes in the brain mRNA expression of neuropeptide y (npy), cocaine-amphetamine regulated transcript (cart), peptide yy (pyy), two isoforms of agouti-related protein (agrp), two isoforms of cholecystokinin (cck), and four isoforms of proopiomelanocortin (pomc) were assessed by q-PCR. In the course of 24 h post-feeding (hpf), several of the brain neuropeptides displayed changes in mRNA expression compared to an unfed control group, indicating that food intake and processing affect the regulation of expression of these genes in Atlantic salmon. Expression of cart, cck-l, pomc-a1 and pomc-b all increased within 3 h of feeding, while most of the feed was still in the stomach, suggesting that these neuropeptides play central anorexigenic roles similar to those described in higher vertebrates, including determining meal intervals. On the other hand, the npy and agrp isoforms which have been described as playing orexigenic roles in mammals, showed an opposite response in salmon and both were elevated in the first 3 h after feeding. The different isoforms of cck, agrp and pomc had different mRNA expression patterns, which indicate specific roles related to feeding regulation. The minimal effect of feeding and digestion on pyy expression in the brain indicates that PYY plays a minor role in the central control of short-term food intake in Atlantic salmon.

► Changes in appetite related neuropeptides in Atlantic salmon brain after feeding.
► Cart, cck-l, pomc-a1 and pomc-b expression increase within 3 hours after feeding.
► Npy and agrp expression is elevated in the postprandial phase.
► Pyy show only minimal response to feeding.
► We suggest an anorexigenic role for cck-l, pomc-a1 and pomc-b similar to mammals.
► While npy and agrp respond opposite to the orexigenic role described in mammals.