کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2801642 1156170 2008 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Female-enriched expression of ERα during gonad differentiation of the urodele amphibian Pleurodeles waltl
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی علوم غدد
پیش نمایش صفحه اول مقاله
Female-enriched expression of ERα during gonad differentiation of the urodele amphibian Pleurodeles waltl
چکیده انگلیسی

In the amphibian Pleurodeles waltl, estradiol treatment of genetically male larvae (ZZ) induces male-to-female sex reversal whereas heat treatment of genetically female larvae (ZW) inhibits estradiol synthesis and leads to female-to-male sex reversal. No data are available on estrogen receptors in this species. In the present study, we have isolated a unique full-length pwERα cDNA and its 5′-flanking region whose promoter activity was confirmed by transfection assays. RT-PCR studies performed in adult animals using ERα-specific primers, revealed that pwERα mRNA was present mainly in reproductive tissues: gonads, fat body and oviduct. PwERα transcript was also detected in liver, suggesting its implication in vitellogenesis control as in numerous oviparous species. The level of pwERα transcripts was also studied during gonad differentiation by quantitative real-time PCR. At stage 5430dpwERα expression in gonads of ZW larvae was significantly higher than in ZZ ones. This sex-specific discrimination was confirmed when gonad–mesonephros–interrenal complexes (GMI), taken at the same stage, were subjected to whole mount in situ hybridization. In comparison, the female-enriched expression of P450 aromatase, which was studied as a control of ovary differentiation, was observed earlier (stage 54). In ZW larvae reared at 32 °C, a condition leading to sex reversal, pwERα mRNA level at stage 5430d was lower than in control females. Taken together, these results showing a female-enriched and thermosensitive expression of pwERα suggest an important role for this receptor in gonad differentiation of the urodele amphibian Pleurodeles waltl.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: General and Comparative Endocrinology - Volume 156, Issue 2, 1 April 2008, Pages 234–245
نویسندگان
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