کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2802240 1156193 2006 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Receptors for neuropeptide Y, γ-aminobutyric acid and dopamine differentially regulate Ca2+ currents in Xenopus melanotrope cells via the Gi protein β/γ-subunit
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی علوم غدد
پیش نمایش صفحه اول مقاله
Receptors for neuropeptide Y, γ-aminobutyric acid and dopamine differentially regulate Ca2+ currents in Xenopus melanotrope cells via the Gi protein β/γ-subunit
چکیده انگلیسی
Secretion of α-melanophore-stimulating hormone (αMSH) from pituitary melanotrope cells of the amphibian Xenopus laevis is under inhibitory synaptic control by three neurotransmitters produced by the suprachiasmatic nucleus: γ-aminobutyric acid (GABA), neuropeptide Y (NPY) and dopamine (DA). These inhibitory effects occur through Gi-protein-coupled receptors (GiPCR), and differ in strength: GABAB-receptor-induced inhibition is the weakest, whereas DA (via a D2-receptor) and NPY (via a Y1-receptor) strongly inhibit, with NPY having a long-lasting effect. Previously it was shown that DA inhibits two (R- and N-type channel) of the four voltage-operated Ca2+ channels in the melanotrope, and that only part of this inhibition is mediated by β/γ-subunits of the Gi protein. We here demonstrate that also the Y1- and GABAB-receptor inhibit only part of the total Ca2+ current (ICa), with fast activation and inactivation kinetics. However, GABAB-mediated inhibition is weaker than the inhibitions induced via Y1- and D2-receptors (−21 versus −27% and −30%, respectively). Using a depolarizing pre-pulse protocol it was demonstrated that GABAB-induced inhibition of ICa most likely depends on Gβ/γ-subunit activation whereas Y1- and D2- induced inhibitions are only partially mediated by Gβ/γ-subunits. No differences were found between the Y1- and D2-induced inhibitions. These results imply that activation of different GiPCR inhibits the ICa through different mechanisms, a phenomenon that may underlie the different potencies of the suprachiasmatic neurotransmitters to inhibit αMSH secretion.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: General and Comparative Endocrinology - Volume 145, Issue 2, 15 January 2006, Pages 140-147
نویسندگان
, , , ,