کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2815064 1159848 2016 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Isolation, genome sequencing and functional analysis of two T7-like coliphages of avian pathogenic Escherichia coli
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Isolation, genome sequencing and functional analysis of two T7-like coliphages of avian pathogenic Escherichia coli
چکیده انگلیسی


• P694 and P483, two highly homologous phages, showed different characteristics.
• P694 and P483 have been shown to be part of the normal intestinal environment.
• P694 generated two types of the coding genes of gp0.3, gp1.1 and gp1.2.
• The tail fiber of APEC phage P694 was closely related to two Yersinia phages.

Avian pathogenic Escherichia coli (APEC) causes colibacillosis, which results in significant economic losses to the poultry industry worldwide. Due to the drug residues and increased antibiotic resistance caused by antibiotic use, bacteriophages and other alternative therapeutic agents are expected to control APEC infection in poultry.Two APEC phages, named P483 and P694, were isolated from the feces from the farmers market in China. We then studied their biological properties, and carried out high-throughput genome sequencing and homology analyses of these phages. Assembly results of high-throughput sequencing showed that the structures of both P483 and P694 genomes consist of linear and double-stranded DNA. Results of the electron microscopy and homology analysis revealed that both P483 and P694 belong to T7-like virus which is a member of the Podoviridae family of the Caudovirales order.Comparative genomic analysis showed that most of the predicted proteins of these two phages showed strongest sequence similarity to the Enterobacteria phages BA14 and 285P, Erwinia phage FE44, and Kluyvera phage Kvp1; however, some proteins such as gp0.6a, gp1.7 and gp17 showed lower similarity (< 85%) with the homologs of other phages in the T7 subgroup. We also found some unique characteristics of P483 and P694, such as the two types of the genes of P694 and no lytic activity of P694 against its host bacteria in liquid medium.Our results serve to further our understanding of phage evolution of T7-like coliphages and provide the potential application of the phages as therapeutic agents for the treatment of diseases.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 582, Issue 1, 10 May 2016, Pages 47–58
نویسندگان
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