کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2815466 | 1159872 | 2016 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular cloning and expression analysis of neuregulin 1 (Nrg1) in the hypothalamus of Huoyan goose during different stages of the egg-laying cycle
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کلمات کلیدی
kiloDaltonrRNAERBBNRG1PKCqRT-PCRkDamRNAEGFORFcDNA - cDNADNA complementary to RNA - DNA مکمل RNAReal-time PCR - PCR به موقعmessenger RNA - RNA messengerRibosomal RNA - RNA Ribosomalamino acid(s) - آمینو اسیدهاrapid-amplification of cDNA ends - تکثیر سریع cDNA به پایان می رسدBase pair(s) - جفت پایه (ها)epidermal growth factor - عامل رشد اپیدرمیopen reading frame - قاب خواندن بازRace - مسابقهUntranslated regions - مناطق غیر ترجمهUTR یا untranslated regions - منطقه ترجمه نشدهHypothalamus - هیپوتالاموسpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازProtein kinase C - پروتئین کیناز سی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
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چکیده انگلیسی
Neuregulin 1 (Nrg1) is one of the most active members of the epidermal growth factor (EGF)-like family, which bind to the ErbB tyrosine kinase receptor and play many roles in modulation of synaptic activity, synaptogenesis, GABAergic neurotransmission, neurotransmitter receptor expression and the hormonal control of neuroendocrine reproductive development. In this study, we cloned and characterized the cDNA of goose Nrg1 originating from hypothalamus tissues of Huoyan goose using RACE method, investigated the mRNA expression profiles during different stages of the egg-laying cycle by real-time PCR. Multiple alignments and phylogenetic analyses of the deduced amino acid sequence were conducted using bioinformatics tools. We also determined the profiles of blood serum progesterone, estradiol, FSH and LH content during different egg-laying stages using radioimmunoassay. The cDNA of Nrg1 is consisted of 2061Â bp open reading frame encoding 686 amino acids. The deduced amino acid sequence of goose Nrg1 contains one EGF domain from amino acid residues 224 to 265 and shows a closer genetic relationship to the avian species than to other mammal species. The expression level of Nrg1 mRNA increased from the pre-laying period to the peak-laying period, reached its peak in the peak-laying period, and then decreased in the ceased period. The concentrations of FSH and estradiol in blood serum have the similar changing trend. These results might suggest a potential correlation between Nrg1/ErbB signaling network with the reproductive neuroendocrine of Huoyan goose.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 575, Issue 2, Part 3, 10 January 2016, Pages 725-731
Journal: Gene - Volume 575, Issue 2, Part 3, 10 January 2016, Pages 725-731
نویسندگان
Zhongzan Cao, Dawei Liu, Mei Liu, Ming Gao, Zimo Chen, Zhe Xing, Xiaoying Zhang, Yunhou Yin, Xinhong Luan,