کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2815656 1159884 2015 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Variation in the ovine PRKAG3 gene
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Variation in the ovine PRKAG3 gene
چکیده انگلیسی


• Three nucleotide substitutions were revealed in ovine PRKAG3.
• SNP g.2656 C > T could nominally lead to a substitution of R230W in ovine PRKAG3.
• The R230W substitution appeared to occur only in sheep.
• This is the first report of genetic variation in ovine PRKAG3.

The 5′AMP-activated protein kinase (AMPK) is a heterotrimeric enzyme that controls cellular energy homeostasis in response to environmental or nutritional stress. The PRKAG3 gene (PRKAG3) encodes the γ3 subunit of the AMPK. Variation in this gene has been found to be associated with meat quality traits in pigs. In this study, we used polymerase chain reaction-single stranded conformational polymorphism (PCR-SSCP) to investigate variation in exon 3 and exons 4–6 of ovine PRKAG3. In 160 New Zealand Suffolk sheep, two variant sequences (named a and b) were identified in the exon 3 region of the gene and three variant sequences (named A, B and C) were identified in the exon 4–6 region of the gene, respectively. A total of three nucleotide substitutions were revealed and these were located in intron 4, exon 4 and intron 5, respectively. The nucleotide substitution identified in the exon 4 (g.2656 C > T) could nominally lead to an amino acid substitution of tryptophan to arginine at position 230 (R230W) in ovine PRKAG3. In comparison with the PRKAG3 amino acid sequences in other species, this R230W substitution appeared to occur only in sheep. This is the first report of genetic variation in ovine PRKAG3, and the variation found in this study could be functionally important for AMPK activity, which in turn may affect meat quality traits in sheep.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 567, Issue 2, 10 August 2015, Pages 251–254
نویسندگان
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