کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2815817 1159894 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
CpG islands of hepatitis B virus genome isolated from Chinese patients
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
CpG islands of hepatitis B virus genome isolated from Chinese patients
چکیده انگلیسی


• Lacking CpG islands I and IV might play a role in the relationship between genotype C and higher risks for HCC and HBV-LC.

There are differences in the distribution and length of HBV CpG islands and the viral mutations contribute greatly to the development of HBV-related diseases. However, little is known regarding the effects of such difference and mutations in HBV genotypes B and C sequences on the regulation of HBV gene expression and their clinical outcomes. To study the distribution, length and genetic trait of CpG islands in normal and mutant sequences of HBV genotypes B and C, 320 HBV isolates from Chinese patients were retrieved from GenBank. Programs CLUSTALX 1.83 and MethPrimer were employed to perform multiple sequence alignments and to predict CpG islands, respectively. 72.0% genotype B isolates contained three conventional CpG islands, and 76.1% genotype C only contained CpG islands II and III. 14.6% genotype B and 7.5% genotype C contained three novel CpG islands. In genotype B, lengths of conventional CpG islands between normal and mutant isolates exhibited substantial variations, but in genotype C, those were relatively stable. CpG island II could be “truncated” or “split”. “Truncated” region mutations were associated with structural and functional abnormalities of HBV genes. Rate of “split” CpG island II in genotype B was much higher than that in genotype C. In the majority of isolates from HCC and HBV–ACLF, genotype C lacked CpG island I and novel islands. Distribution, length and genetic trait of CpG islands in HBV genotypes B and C might affect their methylation status, and further affect regulation of HBV gene expression, leading to different clinical outcomes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 561, Issue 2, 1 May 2015, Pages 261–267
نویسندگان
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