کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2816866 | 1159955 | 2013 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Jumping and gliding rodents: Mitogenomic affinities of Pedetidae and Anomaluridae deduced from an RNA-Seq approach Jumping and gliding rodents: Mitogenomic affinities of Pedetidae and Anomaluridae deduced from an RNA-Seq approach](/preview/png/2816866.png)
• We used a next generation sequencing 454 approach to study rodent transcriptomes.
• We identified the mitogenomes of Pedetes and Graphiurus thanks to RNA-Seq.
• Mitochondrial protein-coding genes are differentially expressed.
• Phylogenies showed that Pedetes groups with Anomalurus into Anomaluromorpha.
• Springhares and scaly-tailed flying squirrels are the closest relatives of castors.
An RNA-Seq strategy was used to obtain the complete set of protein-coding mitochondrial genes from two rodent taxa. Thanks to the next generation sequencing (NGS) 454 approach, we determined the complete mitochondrial DNA genome from Graphiurus kelleni (Mammalia: Rodentia: Gliridae) and partial mitogenome from Pedetes capensis (Pedetidae), and compared them with published rodent and outgroup mitogenomes. We finished the mitogenome sequencing by a series of amplicons using conserved PCR primers to fill the gaps corresponding to tRNA, rRNA and control regions. Phylogenetic analyses of the mitogenomes suggest a well-supported rodent phylogeny in agreement with nuclear gene trees. Pedetes groups with Anomalurus into the clade Anomaluromorpha, while Graphiurus branches within the squirrel-related clade. Moreover, Pedetes + Anomalurus branch with Castor into the mouse-related clade. Our study demonstrates the utility of NGS for obtaining new mitochondrial genomes as well as the importance of choosing adequate models of sequence evolution to infer the phylogeny of rodents.
Journal: Gene - Volume 531, Issue 2, 1 December 2013, Pages 388–397