کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2817622 | 1160001 | 2012 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Phenotype and genotype relationship of glutathione peroxidase1 (GPx1) and rs 1800668 variant: The homozygote effect on kinetic parameters Phenotype and genotype relationship of glutathione peroxidase1 (GPx1) and rs 1800668 variant: The homozygote effect on kinetic parameters](/preview/png/2817622.png)
GPx1 is one of the most important enzymes involved in oxidative balance so that, we studied the phenotype and genotype relationship of GPx1 activity and rs 1800668 (C/T) site and also evaluated the changes of GPx1 kinetic parameters in the rs 1800668 homozygotes. One hundred fifty eight subjects were recruited after clinical exams. The rs 1800668 (C/T) genotype distribution was identified using RFLP-PCR method. The hemolysate GPx1 activity was spectrophotometrically measured in a reaction coupled with glutathione reductase (GR). The GPx1 enzyme was purified using gel filtration chromatography with Sephacryl S-300 column and, Kmapp was studied in the rs 1800668 TT and CC homozygotes. The results showed that the GPx1 activity is significantly associated to the rs 1800668 (C/T) genotype distribution (P < 0.05) so that, the GPx1 activity was high among the CC homozygotes (P < 0.03). In addition, Kmapp for TBHP substrate in the TT homozygote (8.48 μM) was higher than the CC homozygote (5.74 μM). We concluded that the C allele within rs 1800668 position is related to the GPx1activity and may be a potential factor involved in development of inflammatory events.
► We studied the role of rs 1800668 site (C/T) on the hemolysate GPx1activity.
► The hemolysate GPx1 activity related directly to the C allele.
► The polymorphic effect was evaluated using kinetic and prediction studies.
Journal: Gene - Volume 505, Issue 1, 15 August 2012, Pages 19–22