کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2818432 | 1569859 | 2011 | 7 صفحه PDF | دانلود رایگان |
TaSnRK2.7, a SnRK2 (sucrose non-fermenting1-related protein kinase 2) member of wheat, confers enhanced multi-stress tolerance in carbohydrate metabolism. Here, the nucleotide diversity of TaSnRK2.7 was investigated in 60 wheat accessions with different stress-tolerant features. One copy, named TaSnRK2.7-B, was detected in 32 accessions, which mainly exhibited stress tolerance. The TaSnRK2.7-B evolutionary dynamics were examined in these cultivars, which have been subjected to population bottlenecks and intensive selection in breeding.Nucleotide diversity (π) in the entire TaSnRK2.7-B region was 0.00148. Sliding-window analysis demonstrated that 3932–4535 bp was a variation-enriched region, covering the eighth intron, the ninth exon and the 3′-flanking region. As predicted, neutrality tests revealed that the TaSnRK2.7-B protein was relatively conservative, and population bottlenecks or purifying selection may have acted on TaSnRK2.7-B. Furthermore, linkage disequilibrium between SNP loci extends across the entire TaSnRK2.7-B region. A SNP marker of TaSnRK2.7-B was developed and mapped on chromosome 2AL flanked by WMC179.4 and WMC401, which were co-located in the same or adjacent chromosome intervals with QTLs for phosphorus utilization efficiency and accumulation efficiency of stem water-soluble carbohydrates.
Journal: Gene - Volume 478, Issues 1–2, 1 June 2011, Pages 28–34