کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2819029 1569899 2009 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Molecular cloning and characterization of the common marmoset huntingtin gene
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Molecular cloning and characterization of the common marmoset huntingtin gene
چکیده انگلیسی

We report here for the first time the isolation and identification of the common marmoset (Callithrix jacchus) huntingtin (Htt) gene, whose ortholog in humans is known to be related to Huntington's disease (HD). A 9396 nucleotide complementary DNA (cDNA) carrying the putative full-length open reading frame of the marmoset Htt gene was identified, and highly conserved nucleotide and amino acid sequences among primates were observed. Based on this data and using tools evaluated for the detection of the marmoset Htt gene, we have demonstrated gene silencing against the expression of endogenous Htt gene in immortalized common marmoset mononuclear cells by means of RNA interference (RNAi). Taken together, the data presented here may assist us in realizing a non-human primate HD model with the common marmoset.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 432, Issues 1–2, 1 March 2009, Pages 60–66
نویسندگان
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