کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2819306 | 1569918 | 2008 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Short 5â²-flanking regions of the Amy gene of Drosophila kikkawai affect amylase gene expression and respond to food environments
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کلمات کلیدی
GREPolyacrylamide - پلی آکریل آمیدKilobase(s) or 1000 bp - 1000 کیلو بایت یا 1000 کیلو بایتAMY - امیanalysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of varianceGene regulation - تنظیم ژنBase pair(s) - جفت پایه (ها)Cis-regulatory element - عنصر نظارتی CISEvolution - فرگشت Gene duplication - مضاعف سازی ژن، تکثیر ژنpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازPlasmid - پلاسمید
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
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چکیده انگلیسی
Evolution of the duplicated genes and regulation in gene expression is of great interest, especially in terms of adaptation. Molecular population genetic and evolutionary studies on the duplicated amylase genes of Drosophila species have suggested that their 5â²-flanking (cis-regulatory) regions play an important role in evolution of these genes. For better understanding of evolution of the duplicated amylase genes and gene expression, we studied functional significance of the Amy1 gene of Drosophila kikkawai using in vitro deletion mutagenesis followed by P-element-mediated germline transformation. We found that a 1.6-kb of the 5â²-flanking region can produce strikingly higher level of larval amylase activity on starch food compared with that on glucose food. We found two cis-regulatory elements, which increase larval amylase activity on starch food. We also found a larval cis-regulatory element, which responds to the food difference. This food-response element is necessary for the function of the element increasing larval activity on starch food. A 5-bp deletion in a putative GRE caused high amylase activity, indicating a cis-regulatory element decreasing amylase activity. These cis-regulatory elements identified in the 5â²-flanking region could be the targets of natural selection.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 412, Issues 1â2, 15 April 2008, Pages 102-109
Journal: Gene - Volume 412, Issues 1â2, 15 April 2008, Pages 102-109
نویسندگان
Nobuyuki Inomata, Shuichi Nakashima,