کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2820138 1569952 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification and expression of novel isoforms of human stromal cell-derived factor 1
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Identification and expression of novel isoforms of human stromal cell-derived factor 1
چکیده انگلیسی

Stromal Cell-derived factor 1 (SDF-1) is a CXC chemokine that binds to the CXCR4 receptor. Recent publication indicates that the SDF-1/CXCR4 signaling pathway plays a pivotal role during development and in many patho-physiological conditions including hematopoiesis, blood vessel formation, cancer metastasis, angiogenesis and HIV infection. Two human SDF-1 isoforms, SDF-1α and SDF-1β, have been reported to date. Here we report the identification of four additional human SDF-1 isoforms derived from alternative splicing events, SDF-1γ, SDF-1δ, SDF-1ε and SDF-1ϕ. These SDF-1 splice variants all share the same first three exons but contain different fourth exons. The human SDF-1 gene spans over 88 kilobase-pairs on chromosome 10. Using the semi-quantitative RT-PCR method, we determined the tissue distribution of these SDF-1 isoforms. SDF-1α and SDF-1β share similar expression patterns and the highest expression were detected in liver, pancreas and spleen. SDF-1γ seems to be the human orthologue of recently isolated rat SDF-1γ, and its expression was only detected in the heart. SDF-1δ expression can be detected in several adult tissues but the highest expression was detected in fetal liver. When transfected into HEK293 cells, all the SDF-1 isoforms can be detected as secreted proteins in the cell culture media. The conditioned media from transfected cells can stimulate cell migration in a CXCR4-dependent manner. These data suggest that the novel SDF-1 splice variants encode functional proteins.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 374, 7 June 2006, Pages 174–179
نویسندگان
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