کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2820287 | 1569950 | 2006 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Expression of the Op18 gene is maintained by the CCAAT-binding transcription factor NF-Y
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کلمات کلیدی
oncoprotein 18FBSnuclear transcription factor YNF-1E2FNF-YC/EBP - C / EBPElectrophoretic mobility shift assay - آزمون تحرک تحرک الکتروفورزStathmin - استاتمینیbase pairs - جفت پایهfetal bovine serum - سرم جنین گاوEMSA یا electrophoretic mobility shift assay - سنجش تغییر تحرک الکتروفورتیکwild type - نوع وحشیpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازCCAAT enhancer binding protein - پروتئین اتصال دهنده تقویت کننده CCAATkilobase - کیلوبایز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
Op18 (Oncoprotein 18, Stathmin) is a mitotic regulator that is highly expressed in many cancers. We have characterized four functional CCAAT boxes in the Op18 gene located at positions: â 980, â 745, â 599 and â 65, relative to the transcriptional start site. NF-Y is a ubiquitously expressed CCAAT-binding transcription factor that regulates a number of cell cycle controlled genes. We have used promoter-reporter assays and mobility shift assays to functionally examine these CCAAT boxes. All sites contribute to the basal expression of Op18, with the sites at â 980 and â 599 being repressive and the sites at â 745 and â 65 being stimulatory. Mobility shift assays indicate that all CCAAT box sites bind factors in nuclear extracts from Hek293. However, only the repressive site at â 599 and the stimulatory site at â 65 are competent to bind NF-Y, suggesting that NF-Y may play a role in promoting both activation and repression of Op18 expression. The NF-Y site at â 65 accounts for greater than 60% of the Op18 gene expression. EMSA competition studies indicate that NF-Y binds with a much higher affinity to the â 65 site than to the â 599 site, suggesting that in asynchronously growing cells NF-Y functions only to stimulate expression through the â 65 binding site. These data suggest that NF-Y is a major transcription factor promoting expression of Op18.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 377, 1 August 2006, Pages 177-185
Journal: Gene - Volume 377, 1 August 2006, Pages 177-185
نویسندگان
Houda Benlhabib, Julio E. Herrera,