کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2820447 | 1569959 | 2006 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Twelve novel C. elegans RNA candidates isolated by two-dimensional polyacrylamide gel electrophoresis
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Twelve novel C. elegans RNA candidates isolated by two-dimensional polyacrylamide gel electrophoresis Twelve novel C. elegans RNA candidates isolated by two-dimensional polyacrylamide gel electrophoresis](/preview/png/2820447.png)
چکیده انگلیسی
C. elegans small RNAs (< 50 nt) were separated by two-dimensional gel electrophoresis (2D-PAGE). cDNAs were prepared from the RNAs extracted from randomly chosen 2D-PAGE spots. Although many cDNA sequences corresponded to parts of known RNAs, twelve novel small RNA candidates were identified: eleven from 2D-PAGE spots of the mixed-stage worm RNA preparation and one from those of the embryonic RNA preparation. These are encoded in the intergenic regions, in the introns of protein-coding genes, in the anti-sense strand of protein-coding sequences and repetitive sequence regions of the genome. None of them showed a characteristic structure of miRNAs, suggesting that they are candidates of other or new classes of RNAs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 365, 3 January 2006, Pages 83–87
Journal: Gene - Volume 365, 3 January 2006, Pages 83–87
نویسندگان
Yusuke Hokii, Akiyoshi Kubo, Takahiro Ogasawara, Yuhkou Nogi, Akito Taneda, Risa Arai, Akira Muto, Chisato Ushida,