کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2822977 | 1570160 | 2016 | 7 صفحه PDF | دانلود رایگان |
• A novel MRV strain was isolated from wild short-nosed fruit bats in China.
• Phylogenetic analyses suggested B/03 is a serotype 1 MRV of reassortment strain.
• Pathogenesis of B/03 strain was tested in BALB/c mice.
Mammalian orthoreoviruses (MRVs) have a wide range of geographic distribution and have been isolated from humans and various animals. This study describes the isolation, molecular characterization and analysis of pathogenicity of MRV variant B/03 from wild short-nosed fruit bats. Negative stain electron microscopy illustrated that the B/03 strain is a non-enveloped icosahedral virus with a diameter of 70 nm. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) migration patterns showed that the B/03 viral genome contains 10 segments in a 3:3:4 arrangement. The isolate belongs to MRV serotype 1 based on S1 gene nucleotide sequence data. BALB/c mice experimentally infected with B/03 virus by intranasal inoculation developed severe respiratory distress with tissue damage and inflammation. Lastly, B/03 virus has an increased transmission risk between bats and humans or animals.
Journal: Infection, Genetics and Evolution - Volume 43, September 2016, Pages 347–353