کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2823203 1161377 2010 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A simple and rapid molecular method for Leptospira species identification
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک بوم شناسی، تکامل، رفتار و سامانه شناسی
پیش نمایش صفحه اول مقاله
A simple and rapid molecular method for Leptospira species identification
چکیده انگلیسی

Serological and DNA-based classification systems only have little correlation. Currently serological and molecular methods for characterizing Leptospira are complex and costly restricting their world-wide distribution and use. Ligation mediated amplification combined with microarray analysis avoids many of these drawbacks. We demonstrated that this approach used in the Check-Points (CP) assay can successfully applied for the generic detection of Leptospira and can discriminate between saprophytic, intermediate and pathogenic species. In addition, the CP assay could unambiguously detect strains of seven pathogenic species and revealed discrepancies in previous speciation and culture collections. The method provides a valuable tool adding to the molecular study of leptospires and their local and global distribution.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Infection, Genetics and Evolution - Volume 10, Issue 7, October 2010, Pages 955–962
نویسندگان
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