Enhanced adaptation of vesicular stomatitis virus in cells infected with vaccinia virus

Infections involving different viruses (multiple infections) are common in nature and can take place between different strains of the same virus or between different virus species, including DNA and RNA viruses. The influence of multiple infections on viral evolution has been previously studied using different populations of the same virus. Here, we took a step forward by studying the evolution of an RNA virus (vesicular stomatitis virus, VSV) in the presence of a resident DNA virus (vaccinia virus, VV). Cell cultures were infected with a constant amount of VV, and VSV was added at four different post-VV-inoculation times and four different population sizes. The results showed that the presence of VV accelerates the adaptation of VSV to a cellular environment, especially at high population sizes. The effect of VV on VSV evolution was stronger when cells were incubated for longer times with VV prior to the addition of VSV. Our results suggest that cooperation between the two viruses rather than competition might be responsible for the enhanced rate of adaptation of VSV. Further studies are needed to discern whether infections involving different viruses could have an increased ability to escape antiviral strategies.