کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2824456 | 1161657 | 2008 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A method for generating sticky-end PCR products which facilitates unidirectional cloning and the one-step assembly of complex DNA constructs
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A method for generating sticky-end PCR products which facilitates unidirectional cloning and the one-step assembly of complex DNA constructs A method for generating sticky-end PCR products which facilitates unidirectional cloning and the one-step assembly of complex DNA constructs](/preview/png/2824456.png)
چکیده انگلیسی
We have developed and tested a method for the restriction enzyme-independent generation of sticky-end PCR products. The method is suitable for use with a proof-reading polymerase such as pfu, or any other heat-stable polymerase which produces a blunt-end product. The technique can be used to achieve unidirectional cloning of PCR products with an efficiency greater than 90%. Because the sequences of the sticky ends are defined by the user and potentially can be of any length, the method can also be exploited for the one-step construction of recombinant plasmids from multiple functional cassettes, without the use of restriction enzymes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plasmid - Volume 59, Issue 3, May 2008, Pages 155–162
Journal: Plasmid - Volume 59, Issue 3, May 2008, Pages 155–162
نویسندگان
Andrew Walker, James Taylor, Duncan Rowe, David Summers,