کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2829432 1162808 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Single particle analysis confirms distal location of subunits NuoL and NuoM in Escherichia coli complex I
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
Single particle analysis confirms distal location of subunits NuoL and NuoM in Escherichia coli complex I
چکیده انگلیسی

Respiratory complex I catalyses the transfer of electrons from NADH to quinone coupled to the translocation of protons across the membrane. The mechanism of coupling and the structure of the complete enzyme are not known. The membrane domain of the complex contains three similar antiporter-like subunits NuoL/M/N, probably involved in proton pumping. We have previously shown that subunits NuoL/M can be removed from the rest of the complex, suggesting their location at the distal end of the membrane domain. Here, using electron microscopy and single particle analysis, we show that subunits NuoL and M jointly occupy a distal half of the membrane domain, separated by about 10 nm from the interface with the peripheral arm. This indicates that coupling mechanism of complex I is likely to involve long range conformational changes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Structural Biology - Volume 159, Issue 2, August 2007, Pages 238–242
نویسندگان
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