کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2829621 | 1162818 | 2006 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: 4Pi microscopy of quantum dot-labeled cellular structures 4Pi microscopy of quantum dot-labeled cellular structures](/preview/png/2829621.png)
The most prominent restrictions of fluorescence microscopy are the limited resolution and the finite signal. Established conventional, confocal, and multiphoton microscopes resolve at best ∼200 nm in the focal plane and only ⩾500 nm in depth. Additionally, organic fluorophores and fluorescent proteins are bleached after 104–105 excitation cycles. To overcome these restrictions, we synergistically combine the 3- to 7-fold improved axial resolution of 4Pi microscopy with the greatly enhanced photostability of semiconductor quantum dots. Co-localization studies of immunolabeled microtubules and mitochondria demonstrate the feasibility of this approach for routine biological measurements. In particular, we visualize the three-dimensional entanglement of the two networks with unprecedented detail.
Journal: Journal of Structural Biology - Volume 156, Issue 3, December 2006, Pages 517–523