Eimeria bovis-induced modulation of the host cell proteome at the meront I stage

The proteome of Eimeria bovis meront I-carrying host cells was analyzed by two-dimensional gel electrophoresis (2DE) at 14 days p.i. and compared to non-infected control cells. A total of 221 protein spots were modulated in their abundance in E. bovis-infected host cells and were subsequently analyzed by matrix-assisted laser desorption ionization time-of-flight mass spectometry (MALDI-TOF-MS). These analyses identified 104 proteins in total with 25 host cell proteins being up-regulated and 79 proteins being down-regulated in E. bovis-infected host cells. Moreover, 20 newly expressed proteins were identified exclusively in E. bovis-infected host cells and were most likely of parasite origin. Parasite-induced differences in protein abundance concerned distinct functional categories, with most proteins being involved in host cell metabolism, cell structure, protein fate and gene transcription. Some of the modulated molecules also indicated regulatory processes on the level of host cell stress response (HSP70, HSP90), host cell apoptosis (caspase 8) and actin elongation/depolymerization (α-actinin-1, gelsonin, tropomodulin-3, transgelin). Since merozoites I were already released shortly after cell sampling, the current data reflect the situation at the end of first merogony. This is the first proteomic approach on E. bovis-infected host cells that was undertaken to gain a rather broad insight into Eimeria-induced host cell modulation. The data processed in this investigation should provide a useful basis for more detailed analyses concerning Eimeria–host cell interactions.

Whole proteome analyses of Eimeria bovis-infected host endothelial cells at the meront I stage and comprehensive studies of up- and down-regulated host cell proteins.Figure optionsDownload high-quality image (85 K)Download as PowerPoint slideResearch highlights▶ Eimeria bovis-induced modulation of host cell proteome at the meront I stage. ▶ Parasite-induced proteins concerned distinct cellular functional categories (host cell metabolism, cell structure, protein fate and gene transcription). ▶ First proteomic approach on Eimeria bovis-infected host cells to gain a broader insight on parasite-host cell interactions.