کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2830024 | 1163345 | 2009 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The heptanucleotide motif GAGACGC is a key component of a cis-acting promoter element that is critical for SnSAG1 expression in Sarcocystis neurona
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کلمات کلیدی
EPMRlucFLuc - FLUCTransfection - ترانسفکشنrapid amplification of cDNA ends - تقویت سریع cDNA به پایان می رسدGene regulation - تنظیم ژنTranscription - رونویسیrenilla luciferase - رگیلا لوسیفرازluciferase - لوسیفرازFirefly luciferase - لوسیفراز فیرفیلیRace - مسابقهEquine protozoal myeloencephalitis - میلوئنسفالیت پروتئوزای اسبSarcocystis neurona - نورون سارکوسیستیسApicomplexa - هاگ داران، اپیکامپلکساPromoter - پروموترCoccidia - کوکیدیا
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: The heptanucleotide motif GAGACGC is a key component of a cis-acting promoter element that is critical for SnSAG1 expression in Sarcocystis neurona The heptanucleotide motif GAGACGC is a key component of a cis-acting promoter element that is critical for SnSAG1 expression in Sarcocystis neurona](/preview/png/2830024.png)
چکیده انگلیسی
The apicomplexan parasite Sarcocystis neurona undergoes a complex process of intracellular development, during which many genes are temporally regulated. The described study was undertaken to begin identifying the basic promoter elements that control gene expression in S. neurona. Sequence analysis of the 5â²-flanking region of five S. neurona genes revealed a conserved heptanucleotide motif GAGACGC that is similar to the WGAGACG motif described upstream of multiple genes in Toxoplasma gondii. The promoter region for the major surface antigen gene SnSAG1, which contains three heptanucleotide motifs within 135 bases of the transcription start site, was dissected by functional analysis using a dual luciferase reporter assay. These analyses revealed that a minimal promoter fragment containing all three motifs was sufficient to drive reporter molecule expression, with the presence and orientation of the 5â²-most heptanucleotide motif being absolutely critical for promoter function. Further studies should help to identify additional sequence elements important for promoter function and for controlling gene expression during intracellular development by this apicomplexan pathogen.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Biochemical Parasitology - Volume 166, Issue 1, July 2009, Pages 85-88
Journal: Molecular and Biochemical Parasitology - Volume 166, Issue 1, July 2009, Pages 85-88
نویسندگان
Rajshekhar Y. Gaji, Daniel K. Howe,