Keywords: MOPr; μ-opioid receptor; GPCR; G protein-coupled receptor; TM; transmembrane domain; BRET; bioluminescence resonance energy transfer; MD; molecular dynamics; HEK 293; human embryonic kidney 293; Rluc; renilla luciferase; GFP; green fluorescent protein; Y
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Keywords: Betaine; Compatible osmolytes; Hyperosmolarity; 5â² untranslated region; Protein synthesis; 48Â S pre-initiation complex; Bcl-2; B-cell CLL/lymphoma 2; FBS; fetal bovine serum; FLuc; firefly luciferase; HSP70; heat shock protein 70; IRES; internal riboso
Keywords: Leishmania; Firefly luciferase; Renilla luciferase; Gene regulation; Translational regulation; Nutrient stress response; Fluc; firefly luciferase; Fluc-BSD; firefly luciferase fusion to blasticidin S deaminase; Rluc; Renilla luciferase; Rluc-PAC; Renilla
Development of a replicon cell line-based high throughput antiviral assay for screening inhibitors of Zika virus
Keywords: Flavivirus; Zika virus; Luciferase; Antiviral screening; Replicon cell line; ZIKV; Zika virus; JEV; Japanese encephalitis virus; MOI; multiplicity of infection; Rluc; Renilla luciferase;
Characterization of a dengue NS4B inhibitor originating from an HCV small molecule library
Keywords: Dengue; Non-structural protein 4B; NS4B; Antiviral compound; HCV; RLuc; Renilla luciferase; 2â²CMC; 2â²-C-methylcytidine;
High-glucose environment increased thrombospondin-1 expression in keratinocytes via DNA hypomethylation
Keywords: TSP1; thrombospondin-1; AGE; advanced glycation end-product; BSA; bovine serum albumin; 5-Aza-dC; 5-Aza-2'-deoxycytidine; MSP; methylation-specific PCR; ROS; reactive oxygen species; PBS; phosphate-buffered saline; PCR; polymerase chain reaction; Rluc; Re
Netrin-1 Protects Hepatocytes Against Cell Death Through Sustained Translation During the Unfolded Protein Response
Keywords: Netrin; UPR; Hepatocyte; Translation; ATF6; activated transcription factor 6; CMV; cytomegalovirus; DAPK; death-associated protein kinase; DMS; dimethyl sulfate; DR; dependence receptor; DTT; dithiothreitol; eIF2α; Eukaryotic translation initiation facto
SGIP1 alters internalization and modulates signaling of activated cannabinoid receptor 1 in a biased manner
Keywords: Seven transmembrane receptors; G-protein coupled receptors; Cannabinoid receptor 1; Protein-protein interactions; Bias signaling; Receptor endocytosis; SGIP1; src homology 3-domain growth factor receptor-bound 2-like (endophilin) interacting protein 1; CC
Systematic analysis of factors influencing observations of biased agonism at the mu-opioid receptor
Keywords: AC; adenylyl cyclase; α-neo; α-neoendorphin; β-arr1 and β-arr2; β-arrestin 1 and 2; BRET; bioluminescence resonance energy transfer; CHO; Chinese hamster ovary; DAMGO; d-Ala2-N-MePhe4-Gly-ol enkephalin; endo; endomorphin; pERK; extracellular signal r
Early growth response-1 facilitates enterovirus 71 replication by direct binding to the viral genome RNA
Keywords: 3â²UTR; 3â²-untranslated region; 5â²UTR; 5â²-untranslated region; CNS; central nervous system; Co-IP; co-immunoprecipitation; CPE; cytopathogenic effect; DMEM; Dulbecco's modified Eagle's medium; EGR1; early growth response-1; eIF4E; eukaryotic initia
Heterodimerization of human orexin receptor 1 and kappa opioid receptor promotes protein kinase A/cAMP-response element binding protein signaling via a Gαs-mediated mechanism
Keywords: GPCR; G-protein-coupled receptor; OX1R; orexin (hypocretin) receptor 1; KOR; kappa opioid receptor; BRET; bioluminescence resonance energy transfer; FRET; fluorescence resonance energy transfer; CREB; cAMP-response element binding protein; PKA; protein ki
Upregulated, 7q21-22 amplicon candidate gene SHFM1 confers oncogenic advantage by suppressing p53 function in gastric cancer
Keywords: GC; Gastric Cancer; CN; Copy Number; GEO; Gene Expression Omnibus; RT-PCR; Reverse Transcription Polymerase Chain Reaction; aCGH; array Comparative Genomic Hybridization; SNP; Single Nucleotide Polymorphism; FLUC; Firefly luciferase; RLUC; Renilla lucifer
Homo- and hetero-oligomerization of β2-adrenergic receptor in receptor trafficking, signaling pathways and receptor pharmacology
Keywords: AR; adrenergic receptor; AT1R; angiotensin II type 1 receptor; Bk2R; bradykinin type 2 receptor; BRET; bioluminescence resonance energy transfer; cAMP; cyclic adenosine monophosphate; CB1R; cannabinoid type 1 receptor; Co-IP; co-immunoprecipitation; CREB;
Biophysical characterization of sites of host adaptive mutation in the influenza A virus RNA polymerase PB2 RNA-binding domain
Keywords: ATCC; American Type Culture Collection; EMSA; electrophoretic mobility shift assays; FAM; fluorescein amidite; FLuc; firefly luciferase; HEK; human embryonic kidney; NMR; nuclear magnetic resonance; RdRp; RNA-dependent RNA polymerase; RLuc; Renilla lucife
5â²-Untranslated region of heat shock protein 70 mRNA drives translation under hypertonic conditions
Keywords: CrPV; Cricket paralysis virus; FBS; fetal bovine serum; FLuc; Photinus pyralis luciferase; HSPs; heat shock proteins; HSP70; heat shock protein 70; IRES; internal ribosome entry site; RLuc; Renilla reniformis luciferase; SNAT-2; sodium-dependent neutral a
Expeditious neutralization assay for human metapneumovirus based on a recombinant virus expressing Renilla luciferase
Keywords: HMPV; human metapneumovirus; HRSV; human respiratory syncytial virus; CPE; cytopathic effect; rHMPV; recombinant HMPV; GFP; green fluorescent protein; NA-GFP; neutralization assay based on GFP expression; mAb; monoclonal antibody; Rluc; Renilla luciferase
A structural perspective of the MAVS-regulatory mechanism on the mitochondrial outer membrane using bioluminescence resonance energy transfer
Keywords: BiFC; bimolecular fluorescence complementation; BRET; bioluminescence resonance energy transfer; CARD; caspase activation and recruitment domain; HCV; hepatitis C virus; HEK; human embryonic kidney; HR1; 4,3 hydrophobic heptad repeat region 1; IFN-β; int
In vitro efficacy of approved and experimental antivirals against novel genotype 3 hepatitis C virus subgenomic replicons
Keywords: HCV; hepatic C virus; GT; genotype; NS; non-structural; DAA; direct-acting antivirals; 2-CMeA; 2-C-methyl adenosine; CsA; cyclosporin A; Rluc; Renilla luciferase; RLU; relative light units; neo; neomycin transferase gene; G418; geneticin; EC50; 50% effect
In vitro activity of human translation initiation factor eIF4B is not affected by phosphomimetic amino acid substitutions S422D and S422E
Keywords: Eukaryotic translation initiation factor eIF4B; Phosphorylation; Ser422Glu; Ser422Asp; PIK3/mTOR signaling pathway; 48S complex reconstitution; eIF; eukaryotic initiation factor; HCV; Hepatitis C Virus; IRES; Internal Ribosome Entry Site; 5â² UTR; 5â² u
D2-like dopamine and β-adrenergic receptors form a signaling complex that integrates Gs- and Gi-mediated regulation of adenylyl cyclase
Keywords: AC; adenylyl cyclase; βAR and β2AR; β-adrenergic and β2-adrenergic receptor(s), respectively; BRET; bioluminescence resonance energy transfer; D2DR; D3DR and D4DR, D2-, D3-, and D4-dopamine receptor(s), respectively; EGFP; enhanced green fluorescent p
Hepatocyte-derived cultured cells with unusual cytoplasmic keratin-rich spheroid bodies
Keywords: MDB; Mallory-Denk body; SB; spheroid body, K8/K18, keratin 8 and keratin 18; IF; intermediate filament; MRT; malignant rhabdoid tumor; EGFP; enhanced green fluorescent protein; Rluc; Renilla luciferase; EM; electron microscopy; DIC; Differential Interfere
The heptanucleotide motif GAGACGC is a key component of a cis-acting promoter element that is critical for SnSAG1 expression in Sarcocystis neurona
Keywords: EPM; equine protozoal myeloencephalitis; RACE; rapid amplification of cDNA ends; Fluc; firefly luciferase; Rluc; renilla luciferase; Sarcocystis neurona; Transfection; Promoter; Gene regulation; Apicomplexa; Coccidia; Transcription; Luciferase;
Suppression of Hepatitis C Virus Replication by Cyclosporin A Is Mediated by Blockade of Cyclophilins
Keywords: BSD; blasticidin S; CsA; cyclosporin A; CsD; cyclosporin D; CypA; cyclophilin A; CypB; cyclophilin B; CypC; cyclophilin C; ER; endoplasmic reticulum; Fluc; firefly luciferase; His; polyhistidine; IRES; internal ribosome entry site; ISRE; interferon stimul