کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8322724 | 1539882 | 2015 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Early growth response-1 facilitates enterovirus 71 replication by direct binding to the viral genome RNA
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کلمات کلیدی
DMEMeIF4EEGR1IRESEV71RIPATCID50qRT-PCRHFMDHEK-293RT-PCRSK-N-SHRlucRNPPCBP1Enterovirus 71CPEmicroRNA-141ORFLPS3′-untranslated region - 3'-ناحیه ترجمه نشده3′UTR - 3'UTR5′UTR - 5'UTRMOI - MEDulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده DulbeccoSmall interfering RNA - RNA تداخل کوچکsiRNA - siRNAradio-immunoprecipitation assay - آزمایش ادرار رادیو ایمنCytopathogenic effect - اثر سیتوپاتوژنیکstandard deviations - انحراف معیارRNA–protein interaction - تعامل RNA-پروتئینViral replication - تکرار ویروسیhand, foot and mouth disease - دست، پا و دهانCNS - دستگاه عصبی مرکزیRhabdomyosarcoma - رابدومیوسارکوماreverse transcription PCR - رونویسی معکوس PCRrenilla luciferase - رگیلا لوسیفرازribonucleoprotein - ریبونولوپروتئینinternal ribosomal entry site - سایت ورودی ریبوزوم داخلیcentral nervous system - سیستم عصبی مرکزیCo-IP - شرکت-IPeukaryotic initiation factor 4E - عامل آغاز کننده یوکاریوتی 4Eopen reading frame - قاب خواندن بازLipopolysaccharides - لیپوپلی ساکارید ها5′-untranslated region - منطقه 5'-غیر مترادفHuman neuroblastoma - نوروبلاستوما انسانیCo-Immunoprecipitation - هم ایمن زداییquantitative real-time PCR - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیearly growth response-1 - پاسخ سریع رشد 1Poliovirus - پلیو ویروسmultiplicity of infection - چندین عفونتhuman embryonic kidney - کلیه جنین انسان
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Enterovirus 71 (EV71) infections can cause hand, foot and mouth disease (HFMD), meningoencephalitis, neonatal sepsis, and even fatal encephalitis in children. Unfortunately, there is currently no effective treatment for EV71 infection due to the lack of understanding of viral replication and infection; and viral infections have emerged as an imperative global hazard. Thus, it is extremely important to understand the mechanism of EV71 replication in order to prevent and control the diseases associated with EV71 infections. Early growth response-1 (EGR1) is a multifunctional transcription factor that regulates diverse biological functions, including inflammation, apoptosis, differentiation, tumorigenesis, and even viral infection. Here, we provide new insight into the role of EV71 infection in regulating EGR1 production; and reveal a novel mechanism by which EGR1 facilitates EV71 replication. We demonstrate that EV71 activates EGR1 expression during infection by stimulating the protein kinase A/protein kinase CÉ/phosphoinositide 3-kinase/Akt (PKA/PKCÉ/PI3K/Akt) cascade. We further reveal that EV71-activated EGR1, in turn, regulates the internal ribosomal entry site (IRES) of EV71 to enhance viral replication. In addition, EGR1 facilitates EV71 replication by binding directly to stem-loops I and IV of EV71 5â²-untranslated region (5â²UTR) with its first two zinc fingers. Moreover, EGR1 protein co-localizes with EV71 RNA in the cytoplasm of infected cells to facilitate viral replication. Our results reveal an important new role of EGR1 in viral infection, provide new insight into the novel mechanism underlying the regulation of EV71 replication, and suggest a potential application of EGR1 in the control of EV71 infection.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 62, May 2015, Pages 36-46
Journal: The International Journal of Biochemistry & Cell Biology - Volume 62, May 2015, Pages 36-46
نویسندگان
Yu Song, Xin Cheng, Xiaoxia Yang, Rong Zhao, Peili Wang, Yang Han, Zhen Luo, Yanhua Cao, Chengliang Zhu, Ying Xiong, Yingle Liu, Kailang Wu, Jianguo Wu,