D2-like dopamine and β-adrenergic receptors form a signaling complex that integrates Gs- and Gi-mediated regulation of adenylyl cyclase

► The fluorescent reagent FlAsH can be used as an acceptor in BRET experiments. ► β2-Adrenergic and D4-dopamine receptors, Gs, Gi and adenylyl cyclase form a complex. ► Gi activation does not cause the Giα subunit to dissociate from the Gβγ heterodimer. ► Brain contains a complex of β-adrenergic and dopamine receptors and adenylyl cyclase.