کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5506348 1400291 2017 20 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cap-independent protein synthesis is enhanced by betaine under hypertonic conditions
ترجمه فارسی عنوان
سنتز پروتئین مستقل سرپوش با بتائین در شرایط هیپرتونیک افزایش می یابد
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی
Protein synthesis is one of the main cellular functions inhibited during hypertonic challenge. The subsequent accumulation of the compatible osmolyte betaine during the later adaptive response allows not only recovery of translation but also its stimulation. In this paper, we show that betaine modulates translation by enhancing the formation of cap-independent 48 S pre-initiation complexes, leaving cap-dependent 48 S pre-initiation complexes basically unchanged. In the presence of betaine, CrPV IRES- and sodium-dependent neutral amino acid transporter-2 (SNAT2) 5′-UTR-driven translation is 2- and 1.5-fold stimulated in MCF7 cells, respectively. Thus, betaine could provide an advantage in translation of messengers coding for proteins implicated in the response of cells to different stressors, which are often recognized by ribosomal 40 S subunit through simplified cap-independent mechanisms.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 483, Issue 3, 12 February 2017, Pages 936-940
نویسندگان
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