|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|1927803||1050260||2016||6 صفحه PDF||سفارش دهید||دانلود رایگان|
• First reported overexpression of AURKA in melanoma.
• Targeting AURKA inhibits melanoma growth in vitro and in vivo.
• Further found miR-137 suppressed cell growth by binding to AURKA 3′UTR.
• Re-expression of AURKA rescued miR-137-mediated suppression.
• miR-137-AURKA axis may be potential therapeutic targets of melanoma.
As an oncogene, aurora kinase A (AURKA) is overexpressed in various types of human cancers. However, the expression and roles of AURKA in malignant melanoma are largely unknown. In this study, a miR-137-AURKA axis was revealed to regulate melanoma growth. We found a significant increase in levels of AURKA in melanoma. Both genetic knockdown and pharmacologic inhibition of AURKA decreased tumor cell growth in vitro and in vivo. Further found that miR-137 reduced AURKA expression through interaction with its 3′ untranslated region (3′UTR) and that miR-137 was negatively correlated with AURKA expression in melanoma specimens. Overexpression of miR-137 decreased cell proliferation and colony formation in vitro. Notably, re-expression of AURKA significantly rescued miR-137-mediated suppression of cell growth and clonality. In summary, these results reveal that miR-137 functions as a tumor suppressor by targeting AURKA, providing new insights into investigation of therapeutic strategies against malignant melanoma.
Journal: Biochemical and Biophysical Research Communications - Volume 475, Issue 3, 1 July 2016, Pages 251–256