کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2830125 | 1163357 | 2008 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Genomic organization and transcription analysis of the 195-bp satellite DNA in Trypanosoma cruzi
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
RHSRT-PCRLTRSIRESatellite DNA - DNA ماهواره ایViper - ادم خائن و بدنهادEST - استexpression sequence tag - ترتیب بیان ترتیبTrypanosoma cruzi - تریپانوزوم کروزیLong terminal repeats - تکرار طولانی مدتTranscription - رونویسیShort interspersed repetitive element - عنصر تکراری مبهم کوتاهLit - لیتreverse transcription-PCR - معکوس رونویسی PCRliver infusion tryptose medium - کپسول مایع تریپتوز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The 195-bp satellite DNA is the most abundant Trypanosoma cruzi repetitive sequence. Here we show by RNA blotting and RT-PCR that 195 SAT is intensely transcribed. We observed a positive correlation between the level of satellite RNA and the abundance of the satellite copies in the genome of T. cruzi strains and that the satellite expression is not developmentally regulated. By analyzing CL Brener individual reads, we estimated that 195 SAT corresponds to approximately 5% of the CL Brener genome. 195 SAT elements were found in only 37 annotated contigs, indicating that a large number of satellite copies were not incorporated into the assembled data. The assembled satellite units are distributed in non-syntenic regions with Trypanosoma brucei and Leishmania major genomes, enriched with surface proteins, retroelements, RHS and hypothetical proteins. Satellite repeats were not observed in annotated subtelomeric regions. We report that 12 satellite sequences are truncated by the retroelement VIPER.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Biochemical Parasitology - Volume 160, Issue 1, July 2008, Pages 60-64
Journal: Molecular and Biochemical Parasitology - Volume 160, Issue 1, July 2008, Pages 60-64
نویسندگان
Camila Martins, Cassio Silva Baptista, Susan Ienne, Gustavo C. Cerqueira, Daniella C. Bartholomeu, Bianca Zingales,