کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2830356 | 1163379 | 2006 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
3â² gene regulatory elements required for expression of the Plasmodium falciparum developmental protein, Pfs25
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: 3â² gene regulatory elements required for expression of the Plasmodium falciparum developmental protein, Pfs25 3â² gene regulatory elements required for expression of the Plasmodium falciparum developmental protein, Pfs25](/preview/png/2830356.png)
چکیده انگلیسی
Development of sexual stage parasites within the mosquito vector is a crucial step in the transmission of Plasmodium parasites. The expression of the P25 and P28 proteins on the surface of Plasmodium parasites in the mosquito midgut is required for development and hence disease transmission. 3â² gene-flanking sequences are essential for expression of these critical proteins but the nucleotide elements required are poorly defined. Transient gene transfection experiments using constructs containing deletions of the 3â² gene-flanking region of the Plasmodium falciparum P25 homologue, pfs25, reveal that elements necessary for protein expression are within 315 nucleotides (nt) of the stop codon. A T-rich region 137-231 nt from the stop codon is required for expression. The nonamer AATAAAATG, 360 nt downstream from the stop codon, enhances expression by 51 percent. Using 3â² RACE analysis, multiple polyadenylation sites from endogenous and plasmid-derived pfs25 transcripts were identified. Dissimilarities between the identified elements and those of metazoans support the hypothesis that definition of P25/28 3â² gene regulatory processes may eventually permit the development of agents which block malaria transmission but are non-toxic to humans.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Biochemical Parasitology - Volume 146, Issue 2, April 2006, Pages 163-172
Journal: Molecular and Biochemical Parasitology - Volume 146, Issue 2, April 2006, Pages 163-172
نویسندگان
Raphael M. Oguariri, Josephine M. Dunn, Linnie M. Golightly,