کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2831181 | 1570728 | 2013 | 6 صفحه PDF | دانلود رایگان |
In this study, we investigated the potential molecular and immunological differences of a recombinant fusion protein (Hybrid-1), comprising of the immunodominant antigens Ag85B and ESAT-6 from Mycobacterium tuberculosis, derived from two different expression systems, namely Mycobacterium smegmatis and Escherichia coli. The fusion protein was successfully expressed and purified from both bacterial hosts and analyzed for any host-dependent post-translational modifications that might affect the immunogenicity of the protein. We investigated the immunogenicity of Hybrid-1 expressed in the two host species in a murine vaccination model, together with a reference standard Hybrid-1 (expressed in E. coli) from the Statens Serum Institut. No evidence of any post-translation modification was found in the M. smegmatis-derived Hybrid-1 fusion protein, nor were there any significant differences in the T-cell responses obtained to the three antigens analyzed. In conclusion, the Hybrid-1 fusion protein was successfully expressed in a homologous expression system using M. smegmatis and this system is worth considering as a primary source for vaccination trials, as it provided protein of excellent yield, stability and free from lipopolysaccharide.
► We investigated a fusion protein (Ag85B–ESAT-6) from Mycobacterium tuberculosis.
► The fusion protein was expressed in M. smegmatis and Escherichia coli.
► No evidence of any molecular difference was found in the protein from the two hosts.
► There was no significant difference in reactivity of T-cells to the proteins.
► M. smegmatis fusion protein is a vaccine candidate of excellent yield, free from LPS.
Journal: Molecular Immunology - Volume 54, Issues 3–4, July 2013, Pages 278–283