کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2832488 1163838 2007 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A study of the structural correlates of affinity maturation: Antibody affinity as a function of chemical interactions, structural plasticity and stability
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
A study of the structural correlates of affinity maturation: Antibody affinity as a function of chemical interactions, structural plasticity and stability
چکیده انگلیسی

Mutations introduced in an antibody germline sequence as a result of somatic hypermutation could cause its derivatives to have an altered affinity for its target. Affinity maturation favors the selection of the antibodies which exhibit increased affinity. The mutations in 80 high affinity anti-thyroid peroxidase sequences derived from six germlines were analysed in terms of the physicochemical properties of the replacement residues, namely hydrophilicity, size and polarizability, and charge and polarity, in the context of its position and probable solvent accessibility. The effects of these substitutions were evaluated in terms of the resultant increased chemical interactivity potential of the affinity-matured antibodies relative to the germline. The results of the analysis would be useful in the rational design of antibodies and of other proteins for improved binding properties.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Immunology - Volume 44, Issue 6, February 2007, Pages 1342–1351
نویسندگان
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