کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2832911 1163851 2006 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Genomic organization, gene duplication, and expression analysis of interleukin-1β in channel catfish (Ictalurus punctatus)
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
Genomic organization, gene duplication, and expression analysis of interleukin-1β in channel catfish (Ictalurus punctatus)
چکیده انگلیسی

Interleukin-1β (IL-1β) is one of the pivotal early response pro-inflammatory cytokines that enables organisms to respond to infection and induces a cascade of reactions leading to inflammation. In spite of its importance and two decades of studies in the mammalian species, genes encoding IL-1β were not identified from non-mammalian species until recently. Recent research, particularly with genomic approaches, has led to sequencing of IL-1β from many species. Clinical studies also suggested IL-1β as an immunoregulatory molecule potentially useful for enhancing vaccination. However, no IL-1β genes have been identified from channel catfish, the primary aquaculture species from the United States. In this study, we identified two distinct cDNAs encoding catfish IL-1β. Their encoding genes were identified, sequenced, and characterized. The catfish IL-1β genes were assigned to bacterial artificial chromosome (BAC) clones. Genomic studies indicated that the IL-1β genes were tandemly duplicated on the same chromosome. Phylogenetic analysis of various IL-1β genes indicated the possibility of recent species-specific gene duplications in channel catfish, and perhaps also in swine and carp. Expression analysis indicated that both IL-1β genes were expressed, but exhibited distinct expression profiles in various catfish tissues, and after bacterial infection with Edwardsiella ictaluri.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Immunology - Volume 43, Issue 10, April 2006, Pages 1653–1664
نویسندگان
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