کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2833297 1163866 2007 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The expression pattern of three mast cell specific proteases during mouse development
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
The expression pattern of three mast cell specific proteases during mouse development
چکیده انگلیسی

Mast cell chymases and tryptases are major constituents of mast cell granules exhibiting an intriguing but potentially confusing variety of forms and functions. Thanks to recent genetic and biochemical advances, a clearer picture of phylogenetic and functional relationships in this large group of mammalian enzymes is emerging. While different in vivo and in vitro studies characterize the function of these proteases in adult tissues, only sparse information is available about these enzymes during embryogenesis.In the present study, we followed the expression patterns of three different mast cell proteases – mcpt5 (chymase), mcpt6 and 7 (tryptases) – during the development of the mouse embryo. Using specific probe sets for Northern blot analysis, nested PCR and in situ hybridization we describe the expression patterns of the three proteases in different stages of the developing embryo. Furthermore, we try to localize the expression pattern of the three proteases to a certain region and subset of cells. Based on the spatial and temporal expression patterns of mcpt5, 6 and 7 we hypothesize that these molecules play specific and distinct roles in the prenatal and postnatal stages.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Immunology - Volume 44, Issue 5, February 2007, Pages 732–740
نویسندگان
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