کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2833972 1164275 2013 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Molecular evolution of LysR-type transcriptional regulation in Pseudomonas aeruginosa
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک بوم شناسی، تکامل، رفتار و سامانه شناسی
پیش نمایش صفحه اول مقاله
Molecular evolution of LysR-type transcriptional regulation in Pseudomonas aeruginosa
چکیده انگلیسی

Signal perception and transduction through tightly coordinated circuits is integral to the survival and persistence of microbes in diverse ecological niches. The capacity to adapt to changes in the environment is central to their ability to thrive under adverse circumstances. Signal dependent transcriptional regulators are a key mechanism through which microbes assimilate environmental cues and mediate the appropriate adaptive response. By far the largest class of transcriptional regulator is the LysR-class, which is universally distributed among bacteria, archaea, and even eukaryotic organisms. The number of LysR-Type Transcriptional Regulators (LTTRs) varies among species with one of the largest repertoires encoded in the genome of the nosocomial pathogen Pseudomonas aeruginosa. To understand the evolutionary basis for this, we undertook to analyse the relationship between the LTTRs, both at the species and genus level. Phylogenetic analysis of the complete Pseudomonas LTTR dataset revealed significant cluster patterns based on full length and domain analysis. Interestingly, evidence of acquisition through horizontal gene transfer was rare, with divergent evolution apparently favoured. Furthermore, genes that appear to have been acquired, as well as those with a non-classical topological arrangement were clustered in distinct groups in the phylogenetic trees, indicating some ancestral association. The conservation within clusters identified in this study will provide a useful platform for future molecular analyses.

Figure optionsDownload as PowerPoint slideHighlights
► We describe sequence relationships between pseudomonad LysR-type Transcriptional Regulators.
► Conservation of function within clusters suggests useful platform for molecular analyses.
► Phylogenetic trees built on full-length and N/C terminal domain P. aeruginosa sequences are congruent.
► We show that divergence rather than acquisition underpins evolution of LTTRs in P. aeruginosa.
► Classical topological arrangement is maintained in the majority of LTTRs in P. aeruginosa.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Phylogenetics and Evolution - Volume 66, Issue 3, March 2013, Pages 1041–1049
نویسندگان
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