کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2836498 | 1570864 | 2010 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Expression and functional characterization of pathogenesis-related protein family 10 gene, PgPR10-2, from Panax ginseng C.A. Meyer
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کلمات کلیدی
qRT-PCR, Quantitative reverse transcription-polymerase chain reaction - qRT-PCR، واکنش زنجیره ای رونویسی معکوس و پلیمریزاکس معکوسAbiotic stress - استرس بی روحPanax ginseng - جینسنگ Panaxribonuclease - ریبونوکلئازORF, open reading frame - قالب خواندن بازPathogens - پاتوژنPathogenesis-related protein - پروتئین مرتبط با پاتوژنز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Expression and functional characterization of pathogenesis-related protein family 10 gene, PgPR10-2, from Panax ginseng C.A. Meyer Expression and functional characterization of pathogenesis-related protein family 10 gene, PgPR10-2, from Panax ginseng C.A. Meyer](/preview/png/2836498.png)
چکیده انگلیسی
A full-length sequence (PgPR10-2) of the most abundant transcript from 14-year-old ginseng EST library was isolated and cloned. PgPR10-2 gene encodes 465 bp open reading frame and its deduced protein contains 155 amino acids. Genomic DNA sequence reveals it contains two exons interrupted by one intron. Expression of PgPR10-2 gene was especially abundant in roots and its transcripts showed differentially upregulated patterns against several given pathogens and abiotic stimuli. Observed enhanced ribonuclease activity and antifungal activity from tobacco transgenic lines suggest that the possible involvement of PgPR10-2 in defense-related mechanism via ribonuclease activity against biotic and abiotic stresses.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Physiological and Molecular Plant Pathology - Volume 74, Issues 5–6, September 2010, Pages 323–329
Journal: Physiological and Molecular Plant Pathology - Volume 74, Issues 5–6, September 2010, Pages 323–329
نویسندگان
Rama Krishna Pulla, Ok Ran Lee, Jun-Gyo In, Yu-Jin Kim, Kalaiselvi Senthil, Deok-Chun Yang,