کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2844125 1571172 2015 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effect of pentobarbital and isoflurane on acute stress response in rat
ترجمه فارسی عنوان
اثر پنتوباربیتال و ایزوفلوران بر پاسخ استرس حاد در موش صحرایی
کلمات کلیدی
بیهوشی، سدیم پنتوباربیتال، ایسفلورین، فشار، پاسخ استرس
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی فیزیولوژی
چکیده انگلیسی


• Anesthesia before sacrificing animals may interfere with stress research.
• I.p. pentobarbital and isoflurane inhalation do not affect brain mRNA levels.
• I.p. injection causes extra stress and interferes with plasma corticosterone.
• Isoflurane inhalation leaves the stress response intact and is ethically optimal.

BackgroundAnesthesia administration before sacrificing animals is a common practice in stress-related studies, but the effect of anesthesia on the results remains understudied. We aimed to reveal the interference of different anesthetics, i.e. intraperitoneal (i.p.) sodium-pentobarbital injection or isoflurane inhalation, with the acute stress responses in rats.MethodsRats were randomly divided into foot shock (FS) and non-stressed control groups, and further grouped according to the sacrificing procedure: direct decapitation, decapitation after i.p. sodium-pentobarbital injection, or isoflurane inhalation. There was also a non-stressed group sacrificed by decapitation following i.p. saline injection. Plasma levels of corticosterone (CORT), testosterone and estradiol, hypothalamic stress-related molecule mRNA expression of corticotropin-releasing hormone, arginine vasopressin and oxytocin, and frontal lobe stress-related molecule mRNA expression of NMDA receptor subunit NR2B, GABAA receptor and the neuronal-type nicotinic acetylcholine receptor were measured.ResultsFS significantly increased plasma CORT levels in direct decapitation and isoflurane groups, while this stress response ‘disappeared’ following i.p. sodium-pentobarbital injection. In control animals, both the injection of saline and pentobarbital caused a significant increase of plasma CORT. Neither the sex hormone levels nor the mRNA expression of stress-related molecules in the brain showed significant differences among the groups.ConclusionThe injection of the anesthetic compound rather than the compound itself may cause extra stress which interferes with the plasma CORT levels, but not with plasma sex hormone levels nor with the brain mRNA expression. Isoflurane inhalation leaves the stress response intact and is also optimal from an ethical point of view.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Physiology & Behavior - Volume 145, 1 June 2015, Pages 118–121
نویسندگان
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