کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2917848 | 1175674 | 2012 | 12 صفحه PDF | دانلود رایگان |
PurposeThe primary purpose of this study was to examine the changes in myocardial oxidative stress during the support of a left ventricular assist device (LVAD).MethodsMyocardial tissue was collected from the lower left ventricle of 15 adult subjects with class IV heart failure (HF) during LVAD placement (n = 9) or LVAD removal (Post-LVAD; n = 6). Each tissue sample was separated into cytosolic and myofibrillar subfractions and analysed for protein content and carbonylation.ResultsThe myofibrillar proteins in the HF subjects had a significantly lower (p = 0.008) level of protein carbonylation when compared to the myofibrillar proteins in Post-LVAD patients at 1.630 ± 0.277 and 3.075 ± 0.413 optical density, respectively. The level of protein carbonylation in myosin and actin were lower in HF (myosin: 1406.22 ± 218.45, actin: 436 ± 79.72 optical density) subjects compared to Post-LVAD (myosin: 2280.5 ± 441.26, actin: 804.67 ± 155.71 optical density) subjects (p = 0.035 and p = 0.018, respectively). However, once the extent of carbonylation in the myosin and actin bands were normalised to the amount of protein content, all significant difference was lost (HF moysin: 1823.89 ± 413.42, Post-LVAD myosin: 1330.33 ± 297.10 optical density, p = 0.199 and HF actin: 3755.78 ± 349.59, Post-LVAD actin: 4402.83 ± 666.51 optical density, p = 0.182). There was no significant difference in the cytosolic subfractions before or after normalisation of protein content.ConclusionCarbonylation is elevated in the myocardium of HF and Post-LVAD subjects and it appears that LVAD support does not affect the level of myocardial oxidative stress.
Journal: Heart, Lung and Circulation - Volume 21, Issue 9, September 2012, Pages 586–597