کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3011870 | 1181775 | 2012 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Identification of Genes Involved in Smooth Muscle Cell Protein Synthesis with Increased Expression in Atheromatous Plaques Associated with Neointimal Hyperplasia after Bare-Metal Stenting: A GENESIS-R Study Identification of Genes Involved in Smooth Muscle Cell Protein Synthesis with Increased Expression in Atheromatous Plaques Associated with Neointimal Hyperplasia after Bare-Metal Stenting: A GENESIS-R Study](/preview/png/3011870.png)
ABSTRACTBackgroundCoronary restenosis is a poorly understood phenomenon that remains a challenge even in the drug-eluting stent era. This study aimed to identify genes involved in the synthesis of structural and functional proteins in smooth muscle cells with increased expression in human atheromatous plaques associated with neointimal hyperplasia after bare-metal stent implantation.MethodsAtheromatous plaques were obtained by directional atherectomy prior to stenting. A gene expression analysis was performed using the Affymetrix GeneChip system. The patients were subjected to intravascular ultrasound six months after the procedure for an in-stent volumetric analysis. The correlation between gene expression in atheromatous plaques and the percentage of in-stent intimal hyperplasia was evaluated.ResultsMost patients were male (85.7%) with a mean age of 60.2 ± 11.4 years; 35.7% were diabetic, and the percentage of in-stent intimal hyperplasia was 29.9 ± 18.7%. There was no change in the percentage of in-stent intimal hyperplasia in patients with or without diabetes (29.5% vs. 30.7%; P = 0.89). There was no correlation between the stent length and the percentage of in-stent intimal hyperplasia (r = − 0.26; P = 0.26), or between the stent diameter and the percentage of in-stent intimal hyperplasia (r = 0.14; P = 0.56). Eight genes related to smooth uscle cell structural and functional protein synthesis had a positive correlation with the percentage of in-stent intimal hyperplasia.ConclusionsDe novo coronary lesions exhibited an increased expression of genes related to the synthesis of structural and functional proteins in smooth muscle cells and were associated with future significant in-stent neointimal hyperplasia, thus emerging as novel therapeutic targets.
RESUMOIdentificação de Genes Envolvidos na Síntese de Proteínas de Células Musculares Lisascom Expressão Aumentada em Placas Ateromatosas Associados a Hiperplasia Neointimal após Implante de Stents Não Farmacológicos: Estudo GENESIS-RIntroduçãoA reestenose coronária é um fenômeno pouco compreendido e que permanece como um desafio mesmo na era dos stents farmacologicos. Este estudo tem como objetivo identificar genes envolvidos na sintese de proteinas estruturais e funcionais de celulas musculares lisas com expressao aumentada em placas ateromatosas de humanos associados a hiperplasia neointimal apos o implante de stents nao farmacologicos.MétodosPlacas ateromatosas foram obtidas mediante aterectomia direcionada, previamente ao implante do stent. A analise da expressao dos genes foi realizada utilizando-se o sistema Affymetrix GeneChip. Os pacientes foram submetidos a ultrassom intracoronario 6 meses apos o procedimento para analise volumetrica intrastent. Foi avaliada a correlacao entre a expressao genica de placas ateromatosas e o porcentual de hiperplasia intimal intrastent.ResultadosA maioria dos pacientes era do sexo masculino (85,7%), com 60,2 ± 11,4 anos de idade, 35,7% eram diabeticos e o porcentual de hiperplasia intimal intrastent foi de 29,9 ± 18,7%. Nao houve variacao do porcentual de hiperplasia intimal intrastent entre os pacientes com ou sem diabetes (29,5% vs. 30,7%; P = 0,89). Não houve correlação entre a extensão do stent e o porcentual de hiperplasia intimal intrastent (r = − 0,26; P = 0,26) ou entre o diâmetro do stent e o porcentual de hiperplasia intimal intrastent (r = 0,14; P = 0,56). Oito genes envolvidos na síntese de proteínas estruturais e funcionais de células musculares lisas apresentaram correlação positiva com o porcentual de hiperplasia intimal intrastent.ConclusõesAs lesões coronárias de novo apresentam expressão aumentada de genes relacionados com a síntese de proteínas estruturais e funcionais de células musculares lisas associados a futura hiperplasia neointimal intrastent significativa, surgindo como novos alvos terapêuticos.
Journal: Revista Brasileira de Cardiologia Invasiva (English Edition) - Volume 20, Issue 2, 2012, Pages 140–145