Expression of the V264M TFPI mutant in endothelial cell cultures may involve mRNA stability

TFPIV264M displayed increased protein levels and activity compared to TFPIWT accompanied by an increase in mRNA levels of TFPIV264M due to prolonged stability of TFPIV264M mRNA. The specific activity of the TFPIV264M was similar to TFPIWT, indicating that the mutation does not affect the enzymatic function of the protein.