A potential thrombus diagnosis reagent based on P-selectin monoclonal antibody SZ-51 light chain

The yield of SZ-LC by the expression and purification method reached above 70 mg/L culture. We found that the nucleotide from 99mTc-SZ-LC was removed quickly through animal kidney, and 99mTc-SZ-LC could bind specifically to the activated human platelet in vitro. More importantly, with this recombinant protein, we successfully detected the fresh thrombus that was induced in dog vein. These results suggested that the recombinant SZ-LC expressed by P. pastoris was functional active and a potential reagent for thrombus diagnosis.