کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3034441 1579517 2016 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
δ-Opioid receptors: Pivotal role in intermittent hypoxia-augmentation of cardiac parasympathetic control and plasticity
ترجمه فارسی عنوان
گیرنده های δ اوپیوئیدی: نقش محوری در متناوب هیپوکسی-تقویت کنترل پاراسمپاتیک قلبی و انعطاف پذیری
کلمات کلیدی
AR، گیرنده آدرنرژیک؛ ChT-1، کولین حمل کننده-1؛ IHT، آموزش هیپوکسی متناوب؛ OR، receptor opioid؛ ROS، گونه های اکسیژن واکنشی TH، تیروزین هیدروکسیلاز؛ VAChT، vezicular acetylcholine transporterAcetylcholine؛ Enkephalin؛ GM-1؛ نالتیندر
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب سلولی و مولکولی
چکیده انگلیسی


• IHT induced cardioprotection is associated with an improved autonomic profile.
• IHT augments vagally mediated bradycardia.
• Vagotonic δ1-OR activity is enhanced by IHT at the expense of competing vagolytic δ2-OR activity.
• IHT induces parasympathetic nerve growth in myocardium.
• Adrenergic influence over the heart is attenuated by IHT.

BackgroundIntermittent hypoxia training (IHT) produces robust myocardial protection against ischemia-reperfusion induced infarction and arrhythmias. Blockade of this cardioprotection by antagonism of either β1-adrenergic or δ-opioid receptors (δ-OR) suggests autonomic and/or opioidergic adaptations.PurposeTo test the hypothesis that IHT shifts cardiac autonomic balance toward greater cholinergic and opioidergic influence.MethodsMongrel dogs completed 20 d IHT, non-hypoxic sham training, or IHT with the δ-OR antagonist naltrindole (200 μg/kg sc). The vagolytic effect of the δ-OR agonist met-enkephalin-arg-phe delivered by sinoatrial microdialysis was evaluated following IHT. Sinoatrial, atrial and left ventricular biopsies were analyzed for changes in δ-OR, the neurotrophic monosialoganglioside, GM-1, and cholinergic and adrenergic markers.ResultsIHT enhanced vagal bradycardia vs. sham dogs (P < 0.05), and blunted the δ2-OR mediated vagolytic effect of met-enkephalin-arg-phe. The GM-1 labeled fibers overlapped strongly with cholinergic markers, and IHT increased the intensity of both signals (P < 0.05). IHT increased low and high intensity vesicular acetylcholine transporter labeling of sinoatrial nodal fibers (P < 0.05) suggesting an increase in parasympathetic arborization. IHT reduced select δ-OR labeled fibers in both the atria and sinoatrial node (P < 0.05) consistent with moderation of the vagolytic δ2-OR signaling described above. Furthermore, blockade of δ-OR signaling with naltrindole during IHT increased the protein content of δ-OR (atria and ventricle) and vesicular acetylcholine transporter (atria) vs. sham and untreated IHT groups. IHT also reduced the sympathetic marker, tyrosine hydroxylase in ventricle (P < 0.05).SummaryIHT shifts cardiac autonomic balance in favor of parasympathetic control via adaptations in opioidergic, ganglioside, and adrenergic systems.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Autonomic Neuroscience - Volume 198, July 2016, Pages 38–49
نویسندگان
, , , , , , ,