کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3034681 1579534 2014 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Mass spectrometric determination of prostanoids in rat hypothalamic paraventricular nucleus microdialysates
ترجمه فارسی عنوان
تعیین توزیع طیف سنجی پروانستوئیدها در میکرودیالیسات هسته پروژستیکال هیپوتالاموس موش صحرایی
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب سلولی و مولکولی
چکیده انگلیسی

The hypothalamic paraventricular nucleus (PVN) is one of the most important autonomic control centers in the brain. Several kinds of prostanoids, such as prostaglandin (PG) E2, are considered to act in the PVN as mediators of autonomic responses. In the present study, we used liquid chromatography ion trap tandem mass spectrometry (LC–ITMSn) to simultaneously quantify four prostanoids, thromboxane (Tx) B2, PGE2, PGD2 and 15-deoxy-∆12,14 (15d)-PGJ2 in PVN microdialysates from urethane-anesthetized rats. The quantification limits were estimated to be 0.05 ng/mL for TxB2, 0.025 ng/mL for PGE2, 0.1 ng/mL for PGD2, and 0.5 ng/mL for 15d-PGJ2. The RSD% obtained from all prostanoids was < 15%, indicating an acceptable level of reproducibility. LC–ITMSn analysis of rat PVN microdialysates revealed that TxA2 may play an important role in adrenomedullary outflow evoked by centrally administered N-methyl-d-aspartate, corticotrophin-releasing factor and glucagon-like peptide-1. This is the first study to use LC–ITMSn to analyze prostanoid levels in rat PVN microdialysates. This LC–ITMSn method will be useful for investigating the potential involvement of prostanoids in brain function.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Autonomic Neuroscience - Volume 181, April 2014, Pages 49–54
نویسندگان
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