کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3053075 1579950 2007 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Regulation of extracellular calcium in the hippocampus in vivo during epileptiform activity—Role of astrocytes
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی عصب شناسی
پیش نمایش صفحه اول مقاله
Regulation of extracellular calcium in the hippocampus in vivo during epileptiform activity—Role of astrocytes
چکیده انگلیسی

SummaryAstrocytes have been suggested to regulate the extracellular calcium concentration ([Ca2+]o), but this has not been thoroughly investigated. Adult, male Sprague–Dawley rats were used to record changes in [Ca2+]o in the hippocampus during epileptiform activity. Maximal decreases in [Ca2+]o in CA1 were measured in the pyramidal cell layer during 20 Hz, 20 s stimulus trains to the contralateral CA3 region. Maximal decreases in [Ca2+]o in the dentate gyrus were measured when maximal dentate activation had appeared—irrespective of the location, frequency or duration of the stimulation. Maximal decreases were 36% greater in the dentate gyrus than in CA1. During prolonged discharges, [Ca2+]o recovered partially towards the baseline in both hippocampal regions. To investigate the role of astrocytes, local injections of fluorocitrate (FC), a metabolic toxin selectively taken up by astrocytes, were used. FC (0.1, 0.25 or 0.5 mM FC), but not vehicle (2 μl), caused a small, but significant decrease in the maximal changes in CA1, but an increase in the dentate gyrus. The results suggest that maximal decreases in [Ca2+]o occur in the hippocampus in response to burst firing of neurons and that astrocytes play a minimal role in the regulation of [Ca2+]o during epileptiform activity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Epilepsy Research - Volume 74, Issues 2–3, May 2007, Pages 155–162
نویسندگان
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