Pertussis toxin promotes relapsing–remitting experimental autoimmune encephalomyelitis in Lewis rats

• We induced EAE in Lewis rats by homogenized guinea pig spinal cord along with or without pertussis toxin (PT).
• When PT was used, EAE turned into remitting–relapsing form and worsen the clinical symptoms.
• Pertussis toxin administration leads to higher inflammation and oxidative stress.

Animal models simulate different aspects of human diseases and are essential to get a better understanding of the disease, studying treatments and producing new drugs. Experimental autoimmune encephalomyelitis (EAE) is a preferred model in multiple sclerosis research. Common EAE model in Lewis rats is induced using MBP peptide as a myelin antigen which results in a monophasic disease course. In the present study, EAE was induced in Lewis rats by homogenized guinea pig spinal cord along with or without pertussis toxin (PT). When PT was used, EAE turned into remitting–relapsing form and worsen the clinical symptoms. Higher inflammation and oxidative stress marker gene expression was observed when PT was administrated.

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