Mutant Amyloid-beta-sensitized dendritic cells as Alzheimer's disease vaccine

Vaccines using bone marrow-derived dendritic cells (DCs) sensitized to Aβ 1–42 peptide and other mutant peptides were tested on BALB/c and APPSW transgenic mice. Wild type Aβ 1–42-sensitized DC vaccine (DCSV) produced no response, but all peptides with a T-cell epitope mutation induced antibody responses without inflammation. DCSV with Aβ 1–25 peptide with mutated T-cell epitope failed to induce antibody response, while DCSV with Aβ 1–35 with mutated T-cell epitope produced a strong antibody response. The entire T-cell epitope is required in a DC vaccine to induce antibody response. DCSV with Aβ peptide carrying the entire mutant T-cell epitope may be an appropriate vaccine against AD.