کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3066072 | 1580498 | 2006 | 9 صفحه PDF | دانلود رایگان |
We evaluated the regulation of the major histocompatibility complex class II (MHC II) transactivator (CIITA) gene expression in two microglial cell lines, EOC2 and EOC20. We demonstrate that interferon-γ (IFN-γ) activates type III- and IV-CIITA mRNA and high levels of MHC II in EOC20. However, in EOC2 cells only low levels of type IV-CIITA mRNA and MHC II are detectable following IFN-γ treatment. Transforming growth factor-β1 (TGF-β1) inhibits both type III- and IV-CIITA expression in EOC20 cells while, in EOC2 cells TGF-β1 enhances IFN-γ induced pIV-CIITA expression. Trichostatin A (TSA), a histone deacetylase (HDAC) inhibitor, abrogates the TGF-β1 mediated repression of the IFN-γ induced CIITA in EOC20. Evidence is presented that the TG-interacting factor (TGIF), a co-repressor known to recruit HDACs, plays a role in determining the effects of TGF-β1 on microglial cells.
Journal: Journal of Neuroimmunology - Volume 172, Issues 1–2, March 2006, Pages 18–26